Finely selected
NTS (240) were transferred to U-bottom 96 well assay plates containing 200 l of M199 medium supplemented with 5.5 mM D-glucose, penicillin-streptomycin and 5% heat inactivated fetal bovine serum and incubated at 37 C in a 5% CO 2 incubator overnight
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[65] Deficiency of Selenium in blood can lead to subfertility, fetal defects, ovarian cysts, miscarriages, still births, unexplained fertility, premature ovarian failure, lower embryo quality, and decreased number of good oocytes in IVF therapy
